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At 24 h post seeding, medium was removed to waste, and cells were treated with 100 L of metformin (10 mM) or vehicle control (H 2 O) diluted in cRPMI and incubated at 37C in 5% CO 2 /95% humidified air

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RNA isolation and real-time quantitative reverse-transcriptase PCR (qRT-PCR) analysis For RNA extraction, the peqGold RNA isolation kit (Peqlab, Erlangen, Germany) was used for phLF and the RNeasy Mini Plus Kit (Quiagen

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Tricholgia analyzovan pre svoju schopnos stimulova rast vlasov a posilova vlasov folikuly